The analysis relates to polymorphism of the THCA synthase gene, which is the key enzyme in the Δ9-THC pathway. The
determination of hemp genotype belonging to varieties that do not synthesise THCA is based on the absence of the amplification
product of a gene fragment in PCR reaction. The developed method is characterised primarily by the short period of time needed
to obtain results. Additionally, it enables testing of the plant at every stage of its development. The method may contribute to
improving works on breeding industrial hemp varieties with low THC content.
